Transitioning from Triton X-100 to Tergitol 15-S-9: impacts on diagnostic assays using viral PCR sample solution

Transitioning from Triton X-100 to Tergitol 15-S-9: impacts on diagnostic assays using viral PCR sample solution

Blog Article

In 2019, the European Union banned Triton X-100, a detergent widely used Ball - Bag - Backpack in laboratory diagnostics, including the Viral PCR Sample Solution (VPSS), and urged manufacturers to find environmentally sustainable alternatives.Tergitol 15-S-9 (VPSS2) has been proposed as an alternative surfactant.This multicenter study evaluated the effectiveness of VPSS2, a Tergitol-based viral solution, as a replacement for VPSS.Our results show the equivalent performance of VPSS2 to VPSS for nucleic acid extraction and viral stability over time at different temperatures.The Bottom Mount Fridge new VPSS formulation was also tested against external quality assurance panels and clinical samples.

The results of this work support adopting this modified viral PCR sample solution to replace Triton X-100-containing viral transport solutions.